NRG1, isoform SMDF Monoklonaler Antikörper

NRG1, isoform SMDF Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2C6D12

Kat-Nr. : 66492-1-Ig

Synonyme

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF

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Galerie der Validierungsdaten

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  WB analysis using 66492-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of pig cerebellum using 66492-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66492-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66492-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66492-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66492-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Hausschwein-Cerebellum-Gewebe, HepG2-Zellen, Kaninchenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66492-1-Ig bindet in WB, IHC, IF, ELISA NRG1, isoform SMDF und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Vollständiger Name: neuregulin 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007675
• Gene symbol: NRG1
• Gene ID (NCBI): 3084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.

Publikationen

Species	Application	Title
rat	IHC	J Cell Mol Med; PMID: 32073745; Authors - Jianxia Wen
mouse	IF	Int J Mol Sci; Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory; Authors - Harisa Spahic