NRCAM Monoklonaler Antikörper

NRCAM Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E1C3

Kat-Nr. : 68291-1-Ig

Synonyme

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

---

Galerie der Validierungsdaten

• 

  WB analysis using 68291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 68291-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68291-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 68291-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68291-1-Ig bindet in WB, IF, ELISA NRCAM und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NRCAM fusion protein Ag20558
• Vollständiger Name: neuronal cell adhesion molecule
• Berechnetes Molekulargewicht: 1304 aa, 144 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC098401
• Gene symbol: NRCAM
• Gene ID (NCBI): 4897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).