NR2F6 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NR2F6 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2H2B8

Kat-Nr. : 60117-2-Ig

Synonyme

EAR 2, EAR2, ERBAL2, NR2F6, V erbA related protein 2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60117-2-Ig

  WB result of NR2F6 antibody (60117-2-Ig, 1:1000) with si-Control and si-NR2F6 transfected HeLa cells.

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  WB analysis using 60117-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60117-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 60117-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60117-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 60117-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 60117-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-29-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

60117-2-Ig bindet in WB, IHC, ELISA NR2F6 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NR2F6 fusion protein Ag7614
• Vollständiger Name: nuclear receptor subfamily 2, group F, member 6
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC002669
• Gene symbol: NR2F6
• Gene ID (NCBI): 2063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development [PMID:15875024]. NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) [12690040]. It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity [PMID:18701084].

Publikationen

Species	Application	Title
human,mouse	WB	Sci Adv; Melanoma-intrinsic NR2F6 activity regulates antitumor immunity; Authors - Hyungsoo Kim
human	WB	Nat Commun; Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.; Authors - Victoria Klepsch ; KD Validated
human	WB	Biology (Basel); Low-Dose Ionizing Radiation-Crosslinking Immunoprecipitation (LDIR-CLIP) Identified Irradiation-Sensitive RNAs for RNA-Binding Protein HuR-Mediated Decay; Authors - Ji Won Lee