NR1H3 Monoklonaler Antikörper

NR1H3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4A4F7

Kat-Nr. : 60134-1-Ig

Synonyme

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Galerie der Validierungsdaten

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  WB analysis using 60134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60134-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60134-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 60134-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, COLO 320-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

60134-1-Ig bindet in WB, IHC, IF, ELISA NR1H3 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NR1H3 fusion protein Ag6184
• Vollständiger Name: nuclear receptor subfamily 1, group H, member 3
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa 44 kDa
• GenBank-Zugangsnummer: BC041172
• Gene symbol: NR1H3
• Gene ID (NCBI): 10062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.

Publikationen

Species	Application	Title
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang