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NR1D1 Polyklonaler Antikörper

NR1D1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14506-1-AP

Synonyme

ear 1, EAR1, hRev, NR1D1, Rev erbA alpha, Reverb alpha, THRA1, THRAL, V erbA related protein 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Y79-Zellen
Erfolgreiche IPA549-Zellen
Erfolgreiche Detektion in IFNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14506-1-AP bindet in WB, IP, IHC, IF, chIP, ELISA NR1D1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NR1D1 fusion protein Ag5964
Vollständiger Name nuclear receptor subfamily 1, group D, member 1
Berechnetes Molekulargewicht 67 kDa
Beobachtetes Molekulargewicht 55-68 kDa
GenBank-ZugangsnummerBC056148
Gene symbol NR1D1
Gene ID (NCBI) 9572
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR1D1, also named as EAR1, THRAL, ear-1 and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. Rev-erba itself is activated by BMAL1-CLOCK and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Protokolle

Produktspezifische Protokolle
WB protocol for NR1D1 antibody 14506-1-APProtokoll herunterladen
IF protocol for NR1D1 antibody 14506-1-APProtokoll herunterladen
IP protocol for NR1D1 antibody 14506-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu
mouseWB

Theranostics

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

Authors - Shuai Wang
humanWB,IF

Cancer Res

NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer.

Authors - Na-Lee Ka
mouseWB

Cell Death Discov

NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome

Authors - Sun Mi Kim
mouseWB

J Neuroinflammation

The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.

Authors - Liang Kou
humanWB

Cell Biosci

Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.

Authors - Qingxue Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Priya (Verified Customer) (01-17-2023)

I have used for cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin
FH

Satish (Verified Customer) (12-19-2019)

I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: INS1 cells