NPPA Polyklonaler Antikörper

NPPA Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (3)

Kat-Nr. : 27426-1-AP

Synonyme

ANF, ANP, ATFB6, Atrial natriuretic factor, Atrial natriuretic peptide, CDD ANF, CDP, NPPA, PND, Prepronatriodilatin

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Galerie der Validierungsdaten

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  WB analysis using 27426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 27426-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of C2C12 using 27426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 27426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 24 publications below
• ELISA: See 1 publications below

Produktinformation

27426-1-AP bindet in WB, IHC, IF, ELISA NPPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPPA fusion protein Ag26692
• Vollständiger Name: natriuretic peptide precursor A
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC005893
• Gene symbol: NPPA
• Gene ID (NCBI): 4878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.

Publikationen

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p.; Authors - Xudong Zhang
rat	WB	Biochim Biophys Acta Mol Basis Dis; Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ; Authors - Yang Zhou
human,mouse	WB	Pharmacol Res; Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.; Authors - Xingda Li
mouse	WB	ACS Omega; Gastrodin Exerts Cardioprotective Action via Inhibition of Insulin-Like Growth Factor Type 2/Insulin-Like Growth Factor Type 2 Receptor Expression in Cardiac Hypertrophy.; Authors - Jun Lu
mouse	WB	J Cell Physiol; PMID: 30488495; Authors - Jiali Nie
human	WB	Cell Biol Int; HypERlnc attenuates angiotensin II-induced cardiomyocyte hypertrophy via promoting SIRT1 SUMOylation-mediated activation of PGC-1α/PPARα pathway in AC16 cells; Authors - Lijuan Yue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Weak but smear band might need high resolution gels

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

FH

Yan (Verified Customer) (08-19-2020)

It worked when I performed the immunostaining in neonatal mouse ventricular cardiomyocytes culture. Hypertrophy stimulus induced its expression.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: neonatal mouse ventricular cardiomyocytes culture

FH

Jie (Verified Customer) (03-06-2020)

IHC shoes a positive staining in the nuclei

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: cardiomyocyte culture

NPPA Antibody Immunohistochemistry, validation (1:200 dilution) in cardiomyocyte culture (Cat no:27426-1-AP)