Tested Applications
| Positive WB detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22912-1-AP targets Nephrin in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18994 Product name: Recombinant human NPHS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 89-374 aa of BC156935 Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW Predict reactive species |
| Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
| Calculated Molecular Weight | 1241 aa, 135 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC156935 |
| Gene Symbol | Nephrin |
| Gene ID (NCBI) | 4868 |
| RRID | AB_3669406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O60500 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nephrin antibody 22912-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Nephrin Polyclonal antibody (Cat# 22912-1-AP) has 23 citations published in journals including Bioact Mater, Phytomedicine, Journal of Translational Medicine, Apoptosis, Antioxidants, European Journal of Pharmacology, Mitochondrion, Chemical & Biological Interactions, FEBS Journal, Molecular Immunology, Kidney Disease, Journal of Diabetes Research, Experimental Cell Research, Fitoterapia, Journal of Nephrology, International Urology and Nephrology, and Lupus. Citations span research fields of diabetic nephropathy, podocyte biology, renal fibrosis, chronic kidney disease, and nephrotic syndrome.
| Species | Application | Title |
|---|---|---|
Bioact Mater Bioengineered platelet nanoplatform enables renal-targeted dexamethasone delivery for chronic nephritis therapy with dual anti-inflammatory/anti-fibrotic effects and minimized systemic toxicity | ||
Phytomedicine Fangji Huangqi Tang alleviated chronic kidney disease by regulating intestinal bacteria to inhibit the AHR/ROS pathway. | ||
J Transl Med Gut microbiota dysbiosis contributes to diabetic nephropathy via affecting renal lipid deposition and inflammatory responses. | ||
Apoptosis Inhibition of SIRT1/HSF1 pathway contributes to doxorubicin-induced nephrotoxicity in ovarian tumor-bearing mice | ||
Antioxidants (Basel) Identification and Validation of the miR/RAS/RUNX2 Autophagy Regulatory Network in AngII-Induced Hypertensive Nephropathy in MPC5 Cells Treated with Hydrogen Sulfide Donors | ||
J Ethnopharmacol Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin |
