NPC2 Polyklonaler Antikörper

NPC2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 19888-1-AP

Synonyme

HE1, Niemann Pick disease, type C2, NPC2

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Galerie der Validierungsdaten

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  WB analysis using 19888-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19888-1-AP (NPC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 19888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19888-1-AP (NPC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19888-1-AP (NPC2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HT-1080.Zellen, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

19888-1-AP bindet in WB, IHC, ELISA NPC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPC2 fusion protein Ag13719
• Vollständiger Name: Niemann-Pick disease, type C2
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17-21 kDa
• GenBank-Zugangsnummer: BC002532
• Gene symbol: NPC2
• Gene ID (NCBI): 10577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the function of these two proteins within lysosomes are linked closely. NPC2 is also named human epididymis-specific protein 1 (HE1), defects of which are the cause of Niemann-Pick disease type C2, characterized as a lysosomal storage disorder that affects the viscera and the central nervous system. Recent finding suggests that NPC2 may serve as a novel intracrine/autocrine factor that controls adipocyte differentiation and function as well as potential therapeutic target for the treatment of type 2 diabetes and related metabolic disorders.

Publikationen

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
human	WB	Research (Wash D C); Large-Scale CRISPR Screen of LDLR Pathogenic Variants; Authors - Mengjing Li
human,mouse	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded testis were stained for Npc2 (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse testis

NPC2 Antibody Immunofluorescence validation (1:200 dilution) in Mouse testis (Cat no:19888-1-AP)