NOX3-Specific Polyklonaler Antikörper

NOX3-Specific Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 20065-1-AP

Synonyme

GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3, NOX3-Specific

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Galerie der Validierungsdaten

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  WB analysis of human brain using 20065-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20065-1-AP (NOX3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20065-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20065-1-AP (NOX3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

20065-1-AP bindet in WB, ELISA NOX3-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NADPH oxidase 3
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_015718
• Gene symbol: NOX3
• Gene ID (NCBI): 50508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.

Publikationen

Species	Application	Title
mouse	WB	Antioxidants (Basel); Distinction between 2'- and 3'-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.; Authors - Raoul Manuel
mouse	WB	Front Pharmacol; Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway; Authors - Jing Sun
human	WB	Onco Targets Ther; Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.; Authors - Muhammad Zubair Saleem
human	WB	Biomedicines; Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects; Authors - Wenfeng Hu
mouse	WB	J Leukoc Biol; GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing; Authors - Yunxian Dong