NOX2 Polyklonaler Antikörper

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• KD/KO Validated

NOX2 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-19013

Synonyme

CGD, CGD91 phox, CYBB, Cytochrome b 245 heavy chain, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, GP91 1, GP91 PHOX, GP91PHOX, NADPH oxidase 2, NOX2, p22 phagocyte B cytochrome, p91 PHOX

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Galerie der Validierungsdaten

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  FC experiment of RAW 264.7 using CL488-19013

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NOX2 (CL488-19013) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-19013 bindet in FC (Intra) NOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOX2 fusion protein Ag5536
• Vollständiger Name: cytochrome b-245, beta polypeptide
• Berechnetes Molekulargewicht: 577 aa, 65 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032720
• Gene symbol: CYBB
• Gene ID (NCBI): 1536
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reveals the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.