eNOS Polyklonaler Antikörper

eNOS Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 27120-1-AP

Synonyme

cNOS, Constitutive NOS, EC NOS, ECNOS, Endothelial NOS, eNOS, NOS type III, NOS3, NOSIII

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Galerie der Validierungsdaten

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  WB analysis using 27120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27120-1-AP (NOS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27120-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 27120-1-AP (eNOS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, bEnd.3-Zellen, Mausnierengewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 30 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

27120-1-AP bindet in WB, IHC, IF, ELISA eNOS und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: eNOS fusion protein Ag25712
• Vollständiger Name: nitric oxide synthase 3 (endothelial cell)
• Beobachtetes Molekulargewicht: 133 kDa, 69 kDa
• Gene symbol: NOS3
• Gene ID (NCBI): 4846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy; Authors - Lijie Ma
mouse	WB	Redox Biol; Non-steroidal mineralocorticoid receptor antagonist finerenone ameliorates mitochondrial dysfunction via PI3K/Akt/eNOS signaling pathway in diabetic tubulopathy; Authors - Lan Yao
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
human	WB	Int J Biol Macromol; Selection of goat β-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells; Authors - Yulong Wu
rat	WB	Oxid Med Cell Longev; Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way.; Authors - Mengyang Zhang
mouse	WB,IHC	Food Funct; Procyanidins extracted from the litchi pericarp ameliorate atherosclerosis in ApoE knockout mice: their effects on nitric oxide bioavailability and oxidative stress; Authors - Shuang Rong