NOP14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NOP14 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 26854-1-AP

Synonyme

C4orf9, NOL14, NOP14, Nucleolar complex protein 14, Nucleolar protein 14, RES4 25, RES425

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 26854-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 26854-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26854-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 26854-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 26854-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 26854-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 26854-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26854-1-AP (NOP14 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, BxPC-3-Zellen, Mauslungengewebe, MCF-7-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

26854-1-AP bindet in WB, IHC, IF, ELISA NOP14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOP14 fusion protein Ag25476
• Vollständiger Name: NOP14 nucleolar protein homolog (yeast)
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC026035
• Gene symbol: NOP14
• Gene ID (NCBI): 8602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Cell Prolif; CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.; Authors - Yufan Ying ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang