NOL9 Polyklonaler Antikörper

NOL9 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, FRAP

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16083-1-AP

Synonyme

NOL9, nucleolar protein 9

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16083-1-AP bindet in WB, IF, FRAP NOL9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOL9 fusion protein Ag9082
• Vollständiger Name: nucleolar protein 9
• Berechnetes Molekulargewicht: 702 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC009257
• Gene symbol: NOL9
• Gene ID (NCBI): 79707
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.

Publikationen

Species	Application	Title
human	FRAP	Nucleic Acids Res; Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity.; Authors - Witold Szaflarski
human	WB,IF	Mol Biol Cell; LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.; Authors - Castle Christopher D CD