Noggin Polyklonaler Antikörper

Noggin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14772-1-AP

Synonyme

NOG, Noggin, SYM1, SYNS1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14772-1-AP (Noggin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14772-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14772-1-AP (Noggin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14772-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14772-1-AP (Noggin antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

14772-1-AP bindet in WB, IHC, ELISA Noggin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Noggin fusion protein Ag6586
• Vollständiger Name: noggin
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC034027
• Gene symbol: NOG
• Gene ID (NCBI): 9241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14.

What is the molecular weight of Noggin? Is Noggin post-translationally modified?

The molecular weight of Noggin is 26 kDa. Noggin is secreted as a covalently linked glycosylated homodimer (PMID: 12478285).

What is the subcellular localization of Noggin?

Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with HSPG (PMID: 11706034).

What is the tissue expression pattern of Noggin?

In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles (PMID: 21256973).

What is the use of Noggin in cell culture?

Noggin can be used for maintaining the pluripotency of human embryonic stem cells (PMID: 19500111).

Publikationen

Species	Application	Title
mouse	WB	Cell Death Dis; PMID: 29415997; Authors - Shu-Fen Li
hamster	IHC	Birth Defects Res; Cigarette smoke induced neural tube defects by down-regulating noggin expression.; Authors - Chuanhui Yin