RPH3AL Polyklonaler Antikörper

RPH3AL Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15297-1-AP

Synonyme

No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL

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Galerie der Validierungsdaten

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  WB analysis of human liver using 15297-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15297-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15297-1-AP

  IP Result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, L02-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15297-1-AP bindet in WB, IP,ELISA RPH3AL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPH3AL fusion protein Ag7552
• Vollständiger Name: rabphilin 3A-like (without C2 domains)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC005153
• Gene symbol: RPH3AL
• Gene ID (NCBI): 9501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.

Publikationen

Species	Application	Title
human	WB	J Cell Sci; Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.; Authors - Kohichi Matsunaga
human	WB	Clin Chim Acta; Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer.; Authors - Jinn-Shiun Chen