NMUR1 Polyklonaler Antikörper

NMUR1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14619-1-AP

Synonyme

FM 3, FM3, G protein coupled receptor 66, GPC R, GPR66, neuromedin U receptor 1, NMUR1

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Galerie der Validierungsdaten

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  WB analysis of human colon using 14619-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14619-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 14619-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 14619-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug NMUR1 antibody (14619-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, humanes Magengewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14619-1-AP bindet in WB, IHC, FC, ELISA NMUR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMUR1 fusion protein Ag6235
• Vollständiger Name: neuromedin U receptor 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC036543
• Gene symbol: NMUR1
• Gene ID (NCBI): 10316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuromedin-U (NMU) is a widely expressed peptide with the highest abundance in the central nervous system (CNS) and intestinal tract of nearly all vertebrate species. NMU mediates several physiological functions via its two cognate receptors, NMUR1 and NMUR2 (PMID:37102164). NMUR1(NmU receptor 1) has the highest expression in peripheral tissues, particularly the GI tract, pancreas, uterus, and testes but has also been reported in peripheral nerve terminals (PMID:10783389).