NMT1 Monoklonaler Antikörper

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• KD/KO Validated

NMT1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D9B9

Kat-Nr. : 67984-1-Ig

Synonyme

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

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Galerie der Validierungsdaten

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  WB analysis using 67984-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67984-1-Ig (NMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of SKOV-3 using 67984-1-Ig

  WB result of NMT1 antibody (67984-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected SKOV-3 cells.

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  IHC staining of human breast cancer using 67984-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67984-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67984-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen, NIH/3T3-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67984-1-Ig bindet in WB, IHC, ELISA NMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NMT1 fusion protein Ag20541
• Vollständiger Name: N-myristoyltransferase 1
• Berechnetes Molekulargewicht: 496 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC006569
• Gene symbol: NMT1
• Gene ID (NCBI): 4836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.