NME2 Polyklonaler Antikörper

NME2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20493-1-AP

Synonyme

NDK B, NDP kinase B, NDPK B, NDPKB, NME2, puf

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 20493-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20493-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20493-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 20493-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 20493-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

20493-1-AP bindet in WB, IHC, IF, ELISA NME2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NME2 fusion protein Ag13913
• Vollständiger Name: non-metastatic cells 2, protein (NM23B) expressed in
• Berechnetes Molekulargewicht: 152 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC002476
• Gene symbol: NME2
• Gene ID (NCBI): 4831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
rat	WB	Proteomics; FHL2-drived molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.; Authors - Yang Lu
mouse	WB	Biochem Biophys Res Commun; Nicotine induces Nme2-mediated apoptosis in mouse testes.; Authors - Yunqi Gu