- Featured Product
- KD/KO Validated
NLRP3 Polyklonaler Antikörper
NLRP3 Polyklonal Antikörper für FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
FC (Intra)
Konjugation
CoraLite®594 Fluorescent Dye
Kat-Nr. : CL594-19771
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | THP-1-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-19771 bindet in FC (Intra) NLRP3 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | NLR family, pyrin domain containing 3 |
Berechnetes Molekulargewicht | 118 kDa |
Beobachtetes Molekulargewicht | 110 kDa |
GenBank-Zugangsnummer | NM_001127461 |
Gene symbol | NLRP3 |
Gene ID (NCBI) | 114548 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.
Protokolle
Produktspezifische Protokolle | |
---|---|
FC protocol for CL594 NLRP3 antibody CL594-19771 | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |