NLGN1 Monoklonaler Antikörper

NLGN1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2D1A12

Rezensionen (1)

Kat-Nr. : 66964-1-Ig

Synonyme

KIAA1070, neuroligin 1, NLGN1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 66964-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66964-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 66964-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66964-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66964-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66964-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66964-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Cerebellum-Gewebe, Hausschwein-Hirngewebe, humanes Hirngewebe, Kaninchenhirngewebe, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

66964-1-Ig bindet in WB, IHC, ELISA NLGN1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NLGN1 fusion protein Ag6356
• Vollständiger Name: neuroligin 1
• Berechnetes Molekulargewicht: 840 aa, 94 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC032555
• Gene symbol: NLGN1
• Gene ID (NCBI): 22871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).

Publikationen

Species	Application	Title
mouse	WB	Neuron; Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1; Authors - Leon Tejwani
rat	WB	Neuroscience; Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Ji-Yuan Zhao
mouse	WB	J Ethnopharmacol; Multimodal investigation reveals the neuroprotective mechanism of Angong Niuhuang pill for intracerebral hemorrhage: Converging bioinformatics, network pharmacology, and experimental validation; Authors - Jiawei Zhou
human	WB	Aging (Albany NY); Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma; Authors - Sen Qin
mouse	WB	Neurochem Int; Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway; Authors - Tianlin Jiang
mouse	WB	Aging (Albany NY); Longitudinal characterization of behavioral, morphological and transcriptomic changes in a tauopathy mouse model; Authors - Qing Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (02-08-2023)

We used this antibody to assess protein levels in human T-ALL cells by western blot. Loaded 30 ug of protein. We got a good signal with 1:5000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: T cells