TTF1 Monoklonaler Antikörper

TTF1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2C8F3

Kat-Nr. : 66034-1-Ig

Synonyme

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66034-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66034-1-Ig (TTF1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 66034-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TTF1 antibody (66034-1-Ig, Clone: 2C8F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

66034-1-Ig bindet in WB, IHC, IF, ELISA TTF1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NK2 homeobox 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: NM_003317
• Gene symbol: NKX2-1
• Gene ID (NCBI): 7080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).

Publikationen

Species	Application	Title
human	WB	ACS Omega; Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation.; Authors - Kun Wang
human	WB	Cell Biochem Biophys; Suberoylanilide Hydroxamic Acid (SAHA) Reduces Fibrosis Markers and Deactivates Human Stellate Cells via the Epithelial-Mesenchymal Transition (EMT).; Authors - Merve Özel
human	IHC	Nat Commun; Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features; Authors - Weizhu Zhao