TTF1; NKX2-1 Polyklonaler Antikörper

TTF1; NKX2-1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21777-1-AP

Synonyme

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1, TTF1; NKX2-1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21777-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21777-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21777-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21777-1-AP bindet in WB, ELISA TTF1; NKX2-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: NK2 homeobox 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 41-45 kDa
• GenBank-Zugangsnummer: NM_003317
• Gene symbol: NKX2-1
• Gene ID (NCBI): 7080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.

Publikationen

Species	Application	Title
human	WB	Environ Toxicol Pharmacol; Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation; Authors - Qi Xu