NK1.1 (CD161) Monoklonaler Antikörper

NK1.1 (CD161) Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

PK136

Kat-Nr. : 65138-1-Ig

Synonyme

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

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Galerie der Validierungsdaten

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  FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD49b (DX5) and 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

• 

  FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65138-1-Ig bindet in FC NK1.1 (CD161) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NK-1+-Zellen aus Mausmilz und -knochenmark
• Vollständiger Name: killer cell lectin-like receptor subfamily B member 1A
• GenBank-Zugangsnummer: BC120707
• Gene symbol: Klrb1a
• Gene ID (NCBI): 17057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).