NHP2 Polyklonaler Antikörper

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• KD/KO Validated

NHP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 15128-1-AP

Synonyme

NHP2, NHP2P, NOLA2, snoRNP protein NHP2

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 15128-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 15128-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15128-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15128-1-AP

  IP Result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human prostate cancer using 15128-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 15128-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15128-1-AP

  Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Caco-2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15128-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA NHP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hefe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NHP2 fusion protein Ag7251
• Vollständiger Name: NHP2 ribonucleoprotein homolog (yeast)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC000009
• Gene symbol: NHP2
• Gene ID (NCBI): 55651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Publikationen

Species	Application	Title
human	WB	Nature; Structure of human telomerase holoenzyme with bound telomeric DNA.; Authors - George E Ghanim
human	WB	Nature; Cryo-EM structure of substrate-bound human telomerase holoenzyme.; Authors - Thi Hoang Duong Nguyen
human	WB,IF	EMBO J; NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.; Authors - Maya Raghunandan ; KD Validated
human,yeast	WB	EMBO Rep; Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.; Authors - Julia S P Mawer
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB	Mol Cell Biol; SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.; Authors - D E MacNeil