XLF Monoklonaler Antikörper

XLF Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F8C7

Kat-Nr. : 66552-1-Ig

Synonyme

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

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Galerie der Validierungsdaten

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  WB analysis using 66552-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-30000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66552-1-Ig bindet in WB, IHC, ELISA XLF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XLF fusion protein Ag2496
• Vollständiger Name: nonhomologous end-joining factor 1
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC030986
• Gene symbol: NHEJ1
• Gene ID (NCBI): 79840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.