NGRN Polyklonaler Antikörper

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• KD/KO Validated

NGRN Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14885-1-AP

Synonyme

DSC92, FI58G, Neugrin, NGRN

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 14885-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14885-1-AP (NGRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

14885-1-AP bindet in WB, IHC, IF, ELISA NGRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NGRN fusion protein Ag6661
• Vollständiger Name: neugrin, neurite outgrowth associated
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC001682
• Gene symbol: NGRN
• Gene ID (NCBI): 51335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.

Publikationen

Species	Application	Title
human	WB,IF	Cell Metab; A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.; Authors - Jason D Arroyo ; KO Validated
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
		J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart