IkB Alpha Polyklonaler Antikörper

IkB Alpha Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 51066-1-AP

Synonyme

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 51066-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51066-1-AP

  IP Result of anti-IkB alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below

Produktinformation

51066-1-AP bindet in WB, IP, IHC, ELISA IkB Alpha und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC002601
• Gene symbol: NFKBIA
• Gene ID (NCBI): 4792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publikationen

Species	Application	Title
	WB	J Hazard Mater; Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner.; Authors - Daiyang Xia
human	WB	J Immunol; Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation.; Authors - Zheng Zhenhua Z
mouse	WB	Front Immunol; Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages.; Authors - Hao Wang
mouse	WB	Front Immunol; Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.; Authors - Siyuan Chen
mouse	WB	FASEB J; Nintedanib ameliorates osteoarthritis in mice by inhibiting synovial inflammation and fibrosis caused by M1 polarization of synovial macrophages via the MAPK/PI3K-AKT pathway; Authors - Jiangbo Yan
mouse	WB	Nutrients; Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation.; Authors - Mathilde Chataigner