NFATC4 Polyklonaler Antikörper

NFATC4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26311-1-AP

Synonyme

NF AT3, NF ATc4, NFAT3, NFATC4

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Galerie der Validierungsdaten

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  WB analysis using 26311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26311-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26311-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26311-1-AP bindet in WB, IHC, ELISA NFATC4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFATC4 fusion protein Ag24503
• Vollständiger Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 90-105 kDa
• GenBank-Zugangsnummer: BC053855
• Gene symbol: NFATC4
• Gene ID (NCBI): 4776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFATC4, also named NFAT3, is a member from the Nuclear Factor of Activated T cells (NFATs) transcription factor family. NFATc4 contains a conserved Rel homology domain (RHD) which includes the DNA-binding motif, and two transactivation domains (TADs) which locate at the N-terminus and C-terminus respectively. NFATC4 is involved in several physiological processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. It is also reported that NFATC4 could take part in cell growth, cell migration, tumor metastasis, and patient survival recently.