NF90/ILF3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NF90/ILF3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (2)

Kat-Nr. : 19887-1-AP

Synonyme

CBTF, DRBF, DRBP76, ILF3, IL-F3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80

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Galerie der Validierungsdaten

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  WB analysis using 19887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19887-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19887-1-AP (NF90,ILF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19887-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19887-1-AP

  IP Result of anti-ILF3 (IP:19887-1-AP, 3ug; Detection:19887-1-AP 1:1000) with HeLa cells lysate 1600ug.

• 

  IHC staining of human cervical cancer using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19887-1-AP

  Immunofluorescent analysis of Hela cells, using ILF3 antibody 19887-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 4 publications below

Produktinformation

19887-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA NF90/ILF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF90/ILF3 fusion protein Ag13707
• Vollständiger Name: interleukin enhancer binding factor 3, 90kDa
• Berechnetes Molekulargewicht: 894 aa, 95 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 110 kDa
• GenBank-Zugangsnummer: BC064836
• Gene symbol: ILF3
• Gene ID (NCBI): 3609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.

Publikationen

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB	Nucleic Acids Res; Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.; Authors - Aras Siddhesh S

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Praveen (Verified Customer) (03-13-2019)

Very Good

• Applications: Western Blot,
• Cell Tissue Type: HEK293

NF90/ILF3 Antibody Western Blot, validation ( dilution) in HEK293 (Cat no:19887-1-AP)

FH

SHUBHAM (Verified Customer) (02-25-2019)

eXCELLENT

• Applications: Western Blot,
• Cell Tissue Type: Ac16

NF90/ILF3 Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:19887-1-AP)