NF2 Polyklonaler Antikörper

NF2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 21686-1-AP

Synonyme

ACN, BANF, Merlin, Neurofibromin 2, neurofibromin 2 (merlin), NF2, SCH, Schwannomerlin, Schwannomin

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 21686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21686-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21686-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21686-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21686-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21686-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21686-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21686-1-AP

  IP result of anti-NF2(IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug.

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human meningioma using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human meningioma using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21686-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, MCF-7-Zellen, MDCK-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Meningiom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:750-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

21686-1-AP bindet in WB, IP, IHC, IF, ELISA NF2 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF2 fusion protein Ag16265
• Vollständiger Name: neurofibromin 2 (merlin)
• Berechnetes Molekulargewicht: 595 aa, 70 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC020257
• Gene symbol: NF2
• Gene ID (NCBI): 4771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Thyroid; MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.; Authors - Chunxiao Yu
human	WB	J Cell Mol Med; PMID: 32281270; Authors - Jie Liu
human	IF	Onco Targets Ther; PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.; Authors - Lai Wei ; KD Validated
human	WB	Cancer Manag Res; MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.; Authors - Hua-Chun Rao
human	WB	Biochem Biophys Res Commun; A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.; Authors - Yuen Gao
rat	WB	J Cell Physiol; Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.; Authors - Yan-Jing Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bettina (Verified Customer) (08-04-2022)

Worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse & human pancreatic cancer cell lines

NF2 Antibody Western Blot validation (1:1000 dilution) in mouse & human pancreatic cancer cell lines (Cat no:21686-1-AP)