NDUFS5 Polyklonaler Antikörper

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• KD/KO Validated

NDUFS5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15224-1-AP

Synonyme

CI 15 kDa, Complex I 15 kDa, NDUFS5

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Galerie der Validierungsdaten

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  WB analysis using 15224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15224-1-AP

  WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells.

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  IHC staining of human liver using 15224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, L02-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15224-1-AP bindet in WB, IHC, ELISA NDUFS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS5 fusion protein Ag7441
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC001884
• Gene symbol: NDUFS5
• Gene ID (NCBI): 4725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).

Publikationen

Species	Application	Title
human		Genes Dev; ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.; Authors - Yueh-Lin Tsai
mouse	WB	Neurosci Bull; Intermittent Theta Burst Stimulation Attenuates Cognitive Deficits and Alzheimer's Disease-Type Pathologies via ISCA1-Mediated Mitochondrial Modulation in APP/PS1 Mice; Authors - Yang Zhu
mouse	WB	Redox Biol; Regulation of respiratory complex I assembly by FMN cofactor targeting; Authors - Andrea Curtabbi
human	WB	Cell Rep; Regional Molecular Mapping of Primate Synapses during Normal Healthy Aging.; Authors - Laura C Graham