NDUFB8 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFB8 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

3B12C6

Rezensionen (1)

Kat-Nr. : 67690-1-Ig

Synonyme

ASHI, CI ASHI, Complex I ASHI, NDUFB8

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Galerie der Validierungsdaten

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  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67690-1-Ig

  WB result of NDUFB8 antibody (67690-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.

• 

  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

67690-1-Ig bindet in WB, IHC, ELISA NDUFB8 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFB8 fusion protein Ag6569
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC000466
• Gene symbol: NDUFB8
• Gene ID (NCBI): 4714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
mouse	WB	Chin J Nat Med; Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway; Authors - Long Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

Good and strong antibody, but with some non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2, mouse liver and mouse brain

NDUFB8 Antibody Western Blot validation (1:8000 dilution) in HepG2, mouse liver and mouse brain (Cat no:67690-1-Ig)