NDUFB7 Polyklonaler Antikörper

NDUFB7 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14912-1-AP

Synonyme

B18, Cell adhesion protein SQM1, CI B18, Complex I B18, NDUFB7

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14912-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14912-1-AP

  IP Result of anti-NDUFB7 (IP:14912-1-AP, 3ug; Detection:14912-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

14912-1-AP bindet in WB, IP, IHC, ELISA NDUFB7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB7 fusion protein Ag6709
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC002595
• Gene symbol: NDUFB7
• Gene ID (NCBI): 4713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; A disease-associated Aifm1 variant induces severe myopathy in knockin mice.; Authors - Lena Wischhof
mouse	WB	Cell Death Dis; Loss of apoptosis-inducing factor critically affects MIA40 function.; Authors - K Meyer
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
	WB	Mol Med; Altered redox mitochondrial biology in the neurodegenerative disorder fragile X-tremor/ataxia syndrome: use of antioxidants in precision medicine.; Authors - Gyu Song
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang