NDUFB11 Polyklonaler Antikörper

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• KD/KO Validated

NDUFB11 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 16720-1-AP

Synonyme

CI ESSS, Complex I ESSS, ESSS, NDUFB11, Neuronal protein 17.3, P17.3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16720-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16720-1-AP (NDUFB11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16720-1-AP

  IP result of anti-NDUFB11 (IP:16720-1-AP, 4ug; Detection:16720-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000 ug.

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  IHC staining of human kidney using 16720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16720-1-AP (NDUFB11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16720-1-AP (NDUFB11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16720-1-AP

  Immunofluorescent analysis of HepG2 cells using 16720-1-AP (NDUFB11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below

Produktinformation

16720-1-AP bindet in WB, IP, IHC, IF, ELISA NDUFB11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB11 fusion protein Ag10159
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa
• Berechnetes Molekulargewicht: 163 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC107805
• Gene symbol: NDUFB11
• Gene ID (NCBI): 54539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFB11(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial) is also named as neuronal protein 17.3(Np17.3) and belongs to the complex I NDUFB11 subunit family. This protein is involved in the transfer of electrons from NADH to the respiratory chain and play a role in the growth, maintenance, and survival of neurons. NDUFB11 has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	EMBO J; Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.; Authors - Margherita Protasoni
human	WB	Am J Hum Genet; Mutations in NDUFB11, Encoding a Complex I Component of the Mitochondrial Respiratory Chain, Cause Microphthalmia with Linear Skin Defects Syndrome.; Authors - Vanessa A van Rahden ; KD Validated
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.; Authors - Yanchun Ji
		Clin Genet; A novel mutation in NDUFB11 unveils a new clinical phenotype associated with lactic acidosis and sideroblastic anemia.; Authors - Alessandra Torraco
human	WB	Clin Genet; DJ-1 modulates mitochondrial response to oxidative stress: clues from a novel diagnosis of PARK7.; Authors - Michela Di Nottia