NDUFB10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFB10 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 15589-1-AP

Synonyme

CI PDSW, Complex I PDSW, NDUFB10, PDSW

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15589-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 15589-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15589-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15589-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15589-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15589-1-AP (NDUFB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15589-1-AP

  IP Result of anti-NDUFB10 (IP:15589-1-AP, 4ug; Detection:15589-1-AP 1:1000) with HepG2 cells lysate 1000ug.

• 

  IHC staining of human liver using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 15589-1-AP (NDUFB10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15589-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15589-1-AP (NDUFB10 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Skelettmuskelgewebe, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below

Produktinformation

15589-1-AP bindet in WB, IP, IHC, ELISA NDUFB10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB10 fusion protein Ag7922
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC005829
• Gene symbol: NDUFB10
• Gene ID (NCBI): 4716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.

Publikationen

Species	Application	Title
mouse	WB	Cell Stem Cell; LIN28 Regulates Stem Cell Metabolism and Conversion to Primed Pluripotency.; Authors - Jin Zhang
human	WB	Nat Cell Biol; LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.; Authors - Kaloyan M Tsanov
human	WB	Proc Natl Acad Sci U S A; Assembly factors for the membrane arm of human complex I.; Authors - Andrews Byron B
human	WB	Cell Rep; A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.; Authors - Timothy D Martin ; KO Validated
mouse	WB	Front Endocrinol (Lausanne); GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging.; Authors - Bronwen Martin
human	WB	Brain Pathol; Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia.; Authors - Paula Garcia-Esparcia