NDUFA5 Polyklonaler Antikörper

NDUFA5 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16640-1-AP

Synonyme

B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13

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Galerie der Validierungsdaten

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  WB analysis of human brain using 16640-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16640-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16640-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, humanes Herzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

16640-1-AP bindet in WB, IHC, IF, ELISA NDUFA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA5 fusion protein Ag9995
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC000813
• Gene symbol: NDUFA5
• Gene ID (NCBI): 4698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).

Publikationen

Species	Application	Title
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang
human	WB	Cancer Lett; PMID: 29906487; Authors - Linhua Lan
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Proteomics; 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.; Authors - Egan Brendan B
human	WB	Evid Based Complement Alternat Med; Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.; Authors - Jeng-Yuan Shiau
rat	WB	Mol Med Rep; Protein profiling and functional analysis of liver mitochondria from rats with nonalcoholic steatohepatitis.; Authors - Yanting You