NDST1 Polyklonaler Antikörper

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• KD/KO Validated

NDST1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26203-1-AP

Synonyme

HSST, HSST1, N HSST 1, NDST 1, NDST1, NST1

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Galerie der Validierungsdaten

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  WB analysis using 26203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26203-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26203-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26203-1-AP

  IP Result of anti-NDST1 (IP:26203-1-AP, 4ug; Detection:26203-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

26203-1-AP bindet in WB, IP, IF, FC, ELISA NDST1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDST1 fusion protein Ag24422
• Vollständiger Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• Berechnetes Molekulargewicht: 882 aa, 101 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC012888
• Gene symbol: NDST1
• Gene ID (NCBI): 3340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB,IF	Ann Neurol; Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice.; Authors - Yang Huang ; KD Validated
human	WB	PLoS Pathog; N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells.; Authors - Nan Song ; KO Validated
human	WB	Matrix Biol Plus; Glycocalyx mechanotransduction mechanisms are involved in renal cancer metastasis.; Authors - Heriberto Moran
human	WB	J Nanobiotechnology; TSPAN4-positive migrasome derived from retinal pigmented epithelium cells contributes to the development of proliferative vitreoretinopathy; Authors - Liangjing Wu