NDRG1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDRG1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 26902-1-AP

Synonyme

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 26902-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26902-1-AP (NDRG1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HeLa cells.

• 

  WB analysis of HeLa using 26902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26902-1-AP (NDRG1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26902-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDRG1 antibody (26902-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 26902-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NDRG1 (26902-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

26902-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA NDRG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDRG1 fusion protein Ag25424
• Vollständiger Name: N-myc downstream regulated 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC006260
• Gene symbol: NDRG1
• Gene ID (NCBI): 10397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

Publikationen

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	Nucleic Acids Res; TARG1 protects against toxic DNA ADP-ribosylation.; Authors - Callum Tromans-Coia ; KD Validated
human	WB,IHC	Cell Oncol (Dordr); LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity.; Authors - Hao Dang
human	WB	Gynecol Oncol; Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.; Authors - Nan Zhou
human	WB,IHC	PeerJ; LINC00844 promotes proliferation and migration of hepatocellular carcinoma by regulating NDRG1 expression.; Authors - Wei Zhou
human	WB	J Oncol; Ferritinophagic Flux Was a Driving Force in Determination of Status of EMT, Ferroptosis, and NDRG1 Activation in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate S-Acetic Acid.; Authors - Hao Li ; KD Validated