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NDP52 Monoklonaler Antikörper

NDP52 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2C2

Kat-Nr. : CL488-66401

Synonyme

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated NDP52 Monoclonal ant (CL488-66401)

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated NDP52 Monoclonal ant (CL488-66401)

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66401 bindet in IF NDP52 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen NDP52 fusion protein Ag2866
Vollständiger Name calcium binding and coiled-coil domain 2
Berechnetes Molekulargewicht 446 aa, 52 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC015893
Gene symbol CALCOCO2
Gene ID (NCBI) 10241
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 NDP52 antibody CL488-66401Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen