NDP Polyklonaler Antikörper

NDP Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15380-1-AP

Synonyme

EVR2, FEVR, ND, NDP, Norrie disease (pseudoglioma), Norrie disease protein, Norrin

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Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 15380-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 15380-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15380-1-AP bindet in WB,ELISA NDP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDP fusion protein Ag4066
• Vollständiger Name: Norrie disease (pseudoglioma)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC029901
• Gene symbol: NDP
• Gene ID (NCBI): 4693
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDP, also named as EVR2, ND and Norrin, is a secreted regulatory protein that remains tightly associated with the extracellular matrix. Mutations in the NDP gene are associated with the Norrie disease. Signaling induced by the protein NDP regulates vascular development of vertebrate retina and controls important blood vessels in the ear. It binds with high affinity to Frizzled 4, and Frizzled 4 knockout mice exhibit abnormal vascular development of the retina. This antibody detects 11-15 kDa (monomer) and 48 kDa (polymers, refer: 25692504).