NCX1 Polyklonaler Antikörper

NCX1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 55075-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55075-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55075-1-AP (NCX1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55075-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55075-1-AP (NCX1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55075-1-AP

  IP Result of anti-NCX1 (IP:55075-1-AP, 4ug; Detection:55075-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55075-1-AP bindet in WB, IP, IHC, CoIP, ELISA NCX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 8 (sodium/calcium exchanger), member 1
• Berechnetes Molekulargewicht: 109 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_021097
• Gene symbol: SLC8A1
• Gene ID (NCBI): 6546
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	J Am Soc Nephrol; Gentamicin Inhibits Ca2+ Channel TRPV5 and Induces Calciuresis Independent of the Calcium-Sensing Receptor-Claudin-14 Pathway.; Authors - Wouter H van Megen
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao
mouse	IP	J Am Heart Assoc; Sodium-Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt-Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling.; Authors - Yu Zhao
chicken	WB	Chemosphere; NF-κB-mediated inflammation correlates with calcium overload under arsenic trioxide-induced myocardial damage in Gallus gallus.; Authors - Siwen Li
mouse	WB	Alcohol Clin Exp Res; Impact and related mechanisms of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 heterozygous mice.; Authors - Qinfeng Hu
rat	CoIP	Front Physiol; PMID: 32009985; Authors - Raúl Sampieri