Nicastrin Polyklonaler Antikörper

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• KD/KO Validated

Nicastrin Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14071-1-AP

Synonyme

APH2, KIAA0253, NCSTN, nicastrin

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14071-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 14071-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of C6 using 14071-1-AP

  Immunofluorescent analysis of C6 cells using 14071-1-AP (Nicastrin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C6-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

14071-1-AP bindet in WB, IHC, IF, ELISA Nicastrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nicastrin fusion protein Ag5137
• Vollständiger Name: nicastrin
• Berechnetes Molekulargewicht: 709 aa, 78.4 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC047621
• Gene symbol: NCSTN
• Gene ID (NCBI): 23385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

Publikationen

Species	Application	Title
mouse	WB	Gut Microbes; Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis; Authors - Jinghua Jin
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Front Oncol; Stimulation of Let-7 Maturation by Metformin Improved the Response to Tyrosine Kinase Inhibitor Therapy in an m6A Dependent Manner.; Authors - Kai Li
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	WB,IHC	Cancer Cell Int; Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation.; Authors - Xicheng Wang ; KD Validated
mouse	WB	Neurosci Lett; Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.; Authors - Yasuteru Inoue