NCOA6 Polyklonaler Antikörper

NCOA6 Polyklonal Antikörper für FC, IP,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 25241-1-AP

Synonyme

AIB3, ANTP, ASC 2, ASC2, HOX1.1, HOXA7, KIAA0181, NCOA6, NRC, NRC RAP250, nuclear receptor coactivator 6, PPAR interacting protein, PRIP, RAP250, TRBP

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 25241-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25241-1-AP (NCOA6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25241-1-AP

  IP Result of anti-NCOA6 (IP:25241-1-AP, 4ug; Detection:25241-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

• 

  FC experiment of HeLa using 25241-1-AP

  1X10^6 HeLa cells were stained with 0.20ug NCOA6 antibody (25241-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25241-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA NCOA6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCOA6 fusion protein Ag18737
• Vollständiger Name: nuclear receptor coactivator 6
• Berechnetes Molekulargewicht: 2063 aa, 219 kDa
• Beobachtetes Molekulargewicht: 68 kDa, 219 kDa, 110 kDa
• GenBank-Zugangsnummer: BC136272
• Gene symbol: NCOA6
• Gene ID (NCBI): 23054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. The molecular weight of NCOA6 is 219 kDa, but we always detected 68 kDa protein by western blot.

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.; Authors - Yetao Xu
human	WB,IF	Mol Pharmacol; Alterations of Histone Modifications Contribute to Pregnane X Receptor-mediated Induction of CYP3A4 by Rifampicin.; Authors - Liang Yan
human	IHC,CoIP	Cancer Med; Nuclear receptor coactivator 6 (NCoA6) promotes cell proliferation, migration, and invasion in pancreatic cancer; Authors - Xin Wang ; KD Validated