NCOA5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NCOA5 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20175-1-AP

Synonyme

CIA, KIAA1637, NCoA 5, NCOA5, nuclear receptor coactivator 5

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 20175-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20175-1-AP

  IP Result of anti-NCOA5 (IP:20175-1-AP, 4ug; Detection:20175-1-AP 1:800) with HEK-293 cells lysate 3000ug.

• 

  IF Staining of HeLa using 20175-1-AP

  Immunofluorescent analysis of Hela cells, using NCOA5 antibody 20175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HEK-293 using 20175-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug NCOA5 antibody (20175-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

20175-1-AP bindet in WB, IP, IF, FC, ChIP, ELISA NCOA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCOA5 fusion protein Ag14014
• Vollständiger Name: nuclear receptor coactivator 5
• Berechnetes Molekulargewicht: 579 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC056872
• Gene symbol: NCOA5
• Gene ID (NCBI): 57727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; SILAC Proteomics of Planarians Identifies Ncoa5 as a Conserved Component of Pluripotent Stem Cells.; Authors - B?ser Alexander A
bovine	WB,IF,ChIP	Biochem Biophys Res Commun; NCOA5 is a master regulator of amino acid-induced mTOR activation and β-casein synthesis in bovine mammary epithelial cells.; Authors - Xiaohan Yuan ; KD Validated