NOXA2/p67phox Polyklonaler Antikörper

NOXA2/p67phox Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 15551-1-AP

Synonyme

NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, NOXA2/p67phox, P67 PHOX, p67phox

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Galerie der Validierungsdaten

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  WB analysis using 15551-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15551-1-AP (NOXA2/p67phox antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15551-1-AP bindet in WB, IHC, IF, ELISA NOXA2/p67phox und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOXA2/p67phox fusion protein Ag7909
• Vollständiger Name: neutrophil cytosolic factor 2
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC001606
• Gene symbol: NCF2
• Gene ID (NCBI): 4688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
human	WB	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong
mouse	WB,IF	Br J Pharmacol; Rhein regulates redox-mediated activation of NLRP3 inflammasomes in intestinal inflammation through macrophage-activated crosstalk.; Authors - Yangyang Zhou
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang
human	IHC	Front Immunol; Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction; Authors - Hang Ji