NCAPD3 Polyklonaler Antikörper

NCAPD3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16828-1-AP

Synonyme

CAP D3, CAPD3, CNDD3, Condensin 2 complex subunit D3, hCAP D3, hCAP-D3, hcp 6, hHCP 6, KIAA0056, NCAPD3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16828-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16828-1-AP

  IP Result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug.

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  IF Staining of HepG2 using 16828-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

16828-1-AP bindet in WB, IP, IF, ELISA NCAPD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAPD3 fusion protein Ag10429
• Vollständiger Name: non-SMC condensin II complex, subunit D3
• Berechnetes Molekulargewicht: 1498 aa, 169 kDa
• Beobachtetes Molekulargewicht: 165 kDa
• GenBank-Zugangsnummer: BC011408
• Gene symbol: NCAPD3
• Gene ID (NCBI): 23310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).

Publikationen

Species	Application	Title
	WB	Elife; Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays; Authors - Makoto M Yoshida
bovine	IF	Int J Mol Sci; NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility.; Authors - Xin Hu
human	WB	World J Gastroenterol; Non-SMC condensin I complex subunit D2 and non-SMC condensin II complex subunit D3 induces inflammation via the IKK/NF-κB pathway in ulcerative colitis.; Authors - Chang-Wen Yuan
human	WB	Mol Cells; NCAPH Stabilizes GEN1 in Chromatin to Resolve Ultra-Fine DNA Bridges and Maintain Chromosome Stability; Authors - Jae Hyeong Kim
	WB	Mol Biol Cell; Regulation of condensin II by self-suppression and release mechanisms; Authors - Makoto M Yoshida