NAT1 Polyklonaler Antikörper

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• KD/KO Validated

NAT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 19188-1-AP

Synonyme

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 19188-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19188-1-AP

  WB result of NAT1 antibody (19188-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT1 transfected PC-3 cells.

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  WB analysis of human placenta using 19188-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 19188-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 19188-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19188-1-AP

  Immunofluorescent analysis of Hela cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 19188-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, A375-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

19188-1-AP bindet in WB, IHC, IF, CoIP, ELISA NAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAT1 fusion protein Ag6280
• Vollständiger Name: N-acetyltransferase 1 (arylamine N-acetyltransferase)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC047666
• Gene symbol: NAT1
• Gene ID (NCBI): 9
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.; Authors - Rushi Liu
human	WB,CoIP	Nucleic Acids Res; Integrated multi-omics approach revealed cellular senescence landscape; Authors - Qiao Song