NARS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NARS2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15714-1-AP

Synonyme

AsnRS, Asparagine tRNA ligase, NARS2, SLM5

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Galerie der Validierungsdaten

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  WB analysis using 15714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15714-1-AP

  WB result of NARS2 antibody (15714-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NARS2 transfected HepG2 cells.

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  WB analysis of human liver using 15714-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15714-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hirngewebe, humanes Lebergewebe, K-562-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15714-1-AP bindet in WB, IHC, IF,ELISA NARS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NARS2 fusion protein Ag8345
• Vollständiger Name: asparaginyl-tRNA synthetase 2, mitochondrial (putative)
• Berechnetes Molekulargewicht: 477 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC007800
• Gene symbol: NARS2
• Gene ID (NCBI): 79731
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.