NADK Polyklonaler Antikörper

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• KD/KO Validated

NADK Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15548-1-AP

Synonyme

NAD kinase, NADK, Poly(P)/ATP NAD kinase

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 15548-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 15548-1-AP

  Immunofluorescent analysis of Hela cells, using NADK antibody 15548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

15548-1-AP bindet in WB, IHC, IF, CoIP, ELISA NADK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NADK fusion protein Ag7906
• Vollständiger Name: NAD kinase
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC001709
• Gene symbol: NADK
• Gene ID (NCBI): 65220
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

Publikationen

Species	Application	Title
mouse,human	IF	Cell Metab; Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.; Authors - Yang Zhang ; KD Validated
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
	WB	Nucleic Acids Res; Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.; Authors - Shih-Hsun Chen
human	IF	Nat Commun; NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression.; Authors - Chunjing Bian
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin
human	IF	Redox Biol; NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis; Authors - Didem Ilter