N4BP3 Polyklonaler Antikörper

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• KD/KO Validated

N4BP3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 16733-1-AP

Synonyme

KIAA0341, N4BP3, Nedd4 binding protein 3

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 16733-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16733-1-AP (N4BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16733-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16733-1-AP (N4BP3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

16733-1-AP bindet in WB, IP, IHC, ELISA N4BP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: N4BP3 fusion protein Ag10194
• Vollständiger Name: Nedd4 binding protein 3
• Berechnetes Molekulargewicht: 544 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC053323
• Gene symbol: N4BP3
• Gene ID (NCBI): 23138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons. This antibody detects 60 kDa N4BP3 protein and a weaker 40 kDa protein (unknown).

Publikationen

Species	Application	Title
human	WB,IP	Front Microbiol; N4BP3 Regulates RIG-I-Like Receptor Antiviral Signaling Positively by Targeting Mitochondrial Antiviral Signaling Protein.; Authors - Chen Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (05-25-2023)

I used this antibody to assess protein levels in human T-ALL cells by western blot. I got 2 unspecific bands (40 and ~200 kDa). Signal very strong. Dilution: 1:1000 with 50 ug of lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell line